A Comparison Of The Antibacterial Efficiency Of MTAD (Mixture Of Tetracycline, Citric Acid And Detergent), 2.5% Sodium Hypochlorite And 2% Chlorhexidine Root Canal Irrigants Against Enterococcus Faecalis In Root Canals Of Single Rooted Mandibular Premolars- An In Vitro Study

Introduction: Historically, countless compounds in aqueous solution have been suggested as root canal irrigants. MTAD shows promise as an endodontic irrigant but there are very few studies comparing its antimicrobial effectiveness with NaOCl and Chlorhexidine. Objectives: To compare the antibacterial efficiency of MTAD, 2.5% Sodium Hypochlorite and 2% Chlorhexidine root canal irrigants against Enterococcus faecalis in root canals of single rooted mandibular premolars. Method: Inoculum of 10 μl, 24hrs pure culture Enterococcus faecalis was suspended into root canals of forty extracted, decoronated, double sterilized, human mandibular single rooted premolar teeth and incubated for 48hrs at 37°C. Teeth were randomly divided into 4 groups of 10 teeth in each group. The teeth were instrumented in step back technique till size 40 Kfile and irrigated with respective root canal irrigants as per the groups. In Group AMTAD, Group B2.5% NaOCl, Group C2% Chlorhexidine and Group Dnormal saline was used. Bacterial sample from each tooth was collected and bacterial culture was carried out using standard Loop technique. Inoculated plates were incubated at 37°C for 48hrs. Bacterial colonies were counted using colony counter. Statistically Analysed: Using One way ANOVA and LSD Post Hoc test. Results: There was a statistically significant difference in the antibacterial efficiency between all the groups. Conclusion: Within limitations of this study it can be inferred that MTAD has best antibacterial efficiency against Enterococcus faecalis when compared to 2.5% Sodium Hypochlorite and 2% Chlorhexidine.